sc 71 mouse isotype igg1 myhc 2a  (Developmental Studies Hybridoma Bank)

Journal: Experimental and Therapeutic Medicine

Article Title: Morphological and molecular comparisons between tibialis anterior muscle and levator veli palatini muscle: A preliminary study on their augmentation potential

doi: 10.3892/etm.2017.5391

Figure Lengend Snippet: Muscle fiber types in TA and LVP muscle. (A) Immunofluorescence staining of MyHC-1 (red) and laminin (green). Quantification of MyHC-1 is indicated on the right panel. (B) Immunofluorescence staining of MyHC-2A (red) and laminin (green), Quantification of MyHC-2A is indicated on the right panel. (C) Immunofluorescence staining of MyHC-2X (red) and laminin (green). Quantification of MyHC-2X is indicated on the right panel. (D) Immunofluorescence staining of MyHC-2B (red) and laminin (green). Quantification of MyHC-2B is indicated on the right panel. Scale bar=50 µm. ***P<0.001 as indicated. TA, tibialis anterior; LVP, levator veli palatini; MyHC, myosin heavy chain.

Article Snippet: The primary antibodies used were as follows: Rabbit anti-laminin polyclonal antibody (L9393; 1:500; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), mouse anti-Pax7 monoclonal antibody (PAX7; 1:5; Developmental Studies Hybridoma Bank, Iowa City, IA, USA), rabbit anti-Ki67 monoclonal antibody (ab1667; 1:500; Abcam, Cambridge, MA, USA), mouse anti-myosin heavy chain 1 (MyHC-1) monoclonal antibody (A4.840; 1:40; Developmental Studies Hybridoma Bank), mouse anti-MyHC-2A monoclonal antibody (SC-71; 1:20; Developmental Studies Hybridoma Bank), mouse anti-MyHC-2X monoclonal antibody (6H1; 1:5; Developmental Studies Hybridoma Bank) and mouse anti-MyHC-2B monoclonal antibody (BF-F3; 1:5; Developmental Studies Hybridoma Bank).

Techniques: Immunofluorescence, Staining